Comparison of Cold-hardiness Levels of Wheat Cultivars Based on Alterations in Alternative Oxidase Protein Level

Authors : Rahmi DUMLUPINAR, Nilay AKBULUT, Hülya TÜRK, Bagher SHEİKHİ DİDANİ

Pages : 10-15

View : 22 | Download : 5

Publication Date : 2019-12-15

Article Type : Research Paper

Abstract :Plant mitochondrial electron transport chain consists of two terminal oxidases: the cytochrome oxidase insert ignore into journalissuearticles values(COX); and alternative oxidase insert ignore into journalissuearticles values(AOX);. AOX pathway is known to be alternative respiration or cyanide-resistant respiration and linked to stress tolerance of plants. This study aimed to assess cold-hardening levels of four different cultivars of wheat by comparing alterations in protein levels of alternative oxidase, which is responsible for alternative respiration. The AOX protein levels of Yıldırım and Dogu-88 cultivars were markedly higher than those of Ayyıldız and Alpaslan cultivars in control conditions, which indicate that alternative respirations of Yıldırım and Dogu-88 cultivars are more active than those of the other cultivars. Cold stress resulted significant increases AOX protein levels of all cultivars in comparison to their controls. The increases rates were 47, 34, 31, and 70% in Yıldırım, Doğu-88, Ayyıldız, and Alpaslan cultivars, respectively. These findings revealed that although all cultivars tried to resist to cold stress by improving their alternative respirations, Yıldırım and Dogu-88 cultivars are more tolerant to cold stress than the other cultivars.
Keywords : Alternative oxidase, cold stress, wheat, immuno blot analysis