EVALUATION OF EINKORN BRAN AS SUBSTRATE FOR SYNTHESIS OF ALPHA AMYLASE FROM PENICILLIUM HERQUEI IN SOLID STATE FERMENTATION PROCESS

Authors : Seda BALKAN

Pages : 73-76

View : 18 | Download : 11

Publication Date : 2018-11-30

Article Type : Research Paper

Abstract :Normal 0 21 false false false TR X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:`Normal Tablo`; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:``; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:`Calibri`,sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} Solid State Fermentation insert ignore into journalissuearticles values(SSF); is a process defined as microorganisms growing on a solid substrate in the absence of water. It is similar to natural microbial processes such as composting and ensiling. This technique has been known for a long time and is used widely in the Far East.   It can be also used in industrial applications such as Roquefort cheese, antibiotics, organic acids, plant growing agents, food additive materials and enzyme production. In this study, α-amylase production was done in SSF process from Penicillium herquei . Agricultural by-products such as wheat insert ignore into journalissuearticles values( Triticum aestivum L.); bran and einkorn insert ignore into journalissuearticles values( Triticum monococcum L.); bran have been tested for enzyme production, individually and as a mixture. The highest enzyme activity insert ignore into journalissuearticles values(602 U/gds±5.6); was recorded in the SSF process where einkorn bran was used as the substrate. The process parameters such as initial moisture level and substrate amounts were optimized to determine their effects on enzyme synthesis from P. herquei .
Keywords : Solid State Fermentation, einkorn, Penicillium herquei, amylase