KASIB spring common wheat genotype identification on glutenin and gliadin subunits

Authors : Aigul ABUGALİEVA, Alexei MORGOUNOV, Javier Roberto PENA, Nina VOLKOVİNSKAYA, Timur SAVİN

Pages : 32-37

View : 31 | Download : 7

Publication Date : 2015-07-01

Article Type : Research Paper

Abstract :Based on the electrophoretic spectrum, analysis, 216 genotypes of spring wheat were identified as belonging to 25 types including 18 types of HMW-glutenin for 188 homogeneous cultivars. Most of the spring wheat KASIB could be characterized by the subunits 2 * 7+9 5+10 insert ignore into journalissuearticles values(40% genotypes);, and subunits 2* 7+9 2+12– insert ignore into journalissuearticles values(30%); and subunits 1*7+9 5+10– insert ignore into journalissuearticles values(8%);. The diversity appears to be determined by varying chromosome 1A and 1B at the 5 subunits and 1D-4 subunits HMG. Judging from the distribution of genotypes KASIB nurseries are mostly HMW-glutenin determining high quality baking by subunits: 1A-2 *insert ignore into journalissuearticles values(77-79%); and 1 insert ignore into journalissuearticles values(14-15%);, at 1B-7+9 insert ignore into journalissuearticles values(81-82%); and 7+8insert ignore into journalissuearticles values(13-15%);, by 1D –5+10 to 60%. A new subunit is to be included to this set ie., “4+10” for 1D for samples from Erythrospermum 55/9401-20 , Fiton 41 relative to genofund of high-quality genotypes found in Kazakhstan. Cultivar “Iridost” is identified with alleles 5.5+10 on chromosome 1D, previously identified for cultivars Tselinogradka, Tselinnaya 24, Akmola 3. In cultivar Chelyaba, chromosome 1B set possessed only 6+8 subunit and in the mix for cultivars Lutescens 29-94; for genotypes: E-607 and E-757 - a rare characteristic subunit7*+8 was found. Genotypes OK -1, Fiton 25 and GVK 1916-9 carrying wheat-rye 1B/1R-translocation clustered distantly from all other genotypes. Wheat-rye translocation insert ignore into journalissuearticles values(IB/ IR); were identified more in samples for Omsk breeding and West Kazakhstan in each KASIB , for all samples submitted “Kurgansemena” insert ignore into journalissuearticles values(except Lutescens415/00); and “Agrosemkonsalt” , as well as for LLP “Fiton” breeding samples insert ignore into journalissuearticles values(Fiton 41 and Fiton 43, including CIMMYT shuttle breeding material
Keywords : spring wheat, the HMW and the LMW glutenin, gliadin, wheat rye translocation, hardness, varieties identification, UPOV

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