Effect of growth regulators on tissue culture parameters in rice (Oryza sativa L.)

Authors : Berk BENLİOĞLU, Duygu Ege TUNA, Melahat Avcı BİRSİN, Ahmet Murat OZGEN

Pages : 43-46

View : 23 | Download : 8

Publication Date : 2015-07-01

Article Type : Research Paper

Abstract :This study was conducted in Ankara University, Faculty of Agriculture, Department of Field crops, Biotechnology Laboratory. The objective of the present study was to determine the effects of growth regulators on tissue parameters in rice. In this study, mature embryos of three rice cultivars insert ignore into journalissuearticles values(Aromatik-1, Baldo and Karadeniz); and different growth regulators insert ignore into journalissuearticles values(2,4-Dichlorophenoxyacetic acid insert ignore into journalissuearticles values(2,4-D); and picloram); were used as material. For callus induction, mature embryos were placed with scutellum upwards on three different medium insert ignore into journalissuearticles values(hormone-free MS-0, MS + 2 mg/l 2,4-D and MS + 2.5 mg/l picloram); in sterile Petri dishes for two weeks at 25±10C in darkness. After incubation; obtained calli were transferred to hormone-free MS-0 medium for regeneration. According to results, the effect of growth regulators and genotypes on callus induction and plant regeneration in rice were found to be statistically significant
Keywords : rice, oryza sativa L, callus induction, 2, 4 D, picloram