Regeneration of Ruta graveolens Transgenic Plants

Authors : Nadiia A MATVİEİEVA

Pages : 66-71

View : 11 | Download : 7

Publication Date : 2017-01-31

Article Type : Research Paper

Abstract :Here we report the construction of transgenic Ruta graveolens L. plants. The plants were obtained via direct shoot regeneration from leaves and petioles on Murashige and Skoog insert ignore into journalissuearticles values(MS); solidified medium supplemented with 6-benzylaminopurine insert ignore into journalissuearticles values(1.0 mg L-1); and α-naphthylacetic acid insert ignore into journalissuearticles values(0,05 mg L-1); in case of A. tumefaciens-mediated transformation as well as via direct shoot regeneration from rue “hairy” root culture on hormone free half-strength MS medium in case of A. rhizogenes-mediated transformation. Frequency of “hairy” root formation was up to 30%. All transgenic root lines were able to form shoots. Using of A. tumefaciens allowed to obtain transgenic rue plants with high frequency up to 100%. Indolil butyric acid addition to the medium in concentration 0.5 mg L-1 enabled transgenic plant rooting. The shoots regenerated from rue “hairy” root culture were more sensitive to the growth regulator addition and rooted in 2 weeks. Rooting of the shoots obtained after A. tumefaciens-mediated transformation was observed only in 6 weeks under these cultivation conditions.
Keywords : Ruta graveolens L, A tumefaciens, A rhizogenes, genetic transformation, plant regeneration, hairy root culture