The effects of some organic solvents on the modified Ellman procedure for the assay of cholinesterases

Authors : Tugba ERCETİN, Açelya MAVİDENİZ, Karar SHUKUR, Ali KHOSRAVİ, Bahamehr RİAZİ, Fatameh SALAMATİ, Houra SHAHİNFAR, Mohammed NAJJARAN, Muzahem AHMED, Nasim MAHER, Negar KHAJEH, Nezhla DAVDAR, Salva SHAHİNFAR, Hayrettin Ozan GÜLCAN

Pages : 153-158

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Publication Date : 2020-12-31

Article Type : Research Paper

Abstract :Ellman’s method is the most acknowledged procedure in order to determine the cholinesterase inhibitory potential of substances. It is a quick and high-throughput screening method. Therefore, it is widely used to measure the activities of original molecules and mixtures in aqueous solvents through a UV method. Regarding that many original or known substances as well as mixtures require some organic solvent to guarantee dissolution within this methodology, it becomes critical to be aware of the inhibitory potential of organic solvents. From this perspective, within the present study, it was aimed to screen the inhibitory potential of some organic solvents on human acetylcholinesterase and human butyrylcholinesterase enzymes. The results displayed the potent inhibitory function of DMSO. On the other hand, alcohols also pointed out varying degrees of deactivation of the catalytic function of cholinesterases.
Keywords : Organic solvents, acetylcholinesterase, Butyrylcholinesterase, Ellmans method