TLC and GC-MS analysis of fermented wood `Nikhra` petroleum ether fraction of Combretaceae spp. Combretum hartmannianum and Terminalia laxiflora

Authors : Noha FADLE, Abdalbasit MARİOD, Hiba Abdel Rahman ALİ, Alfatih Ahmed HASAN

Pages : 1-7

Doi:10.31195/ejejfs.417112

View : 21 | Download : 11

Publication Date : 2018-10-26

Article Type : Research Paper

Abstract :This study aims to analysis fractions insert ignore into journalissuearticles values( petroleum ether, chloroform, methanol  and aqueous); fermented wood Nikhra of Combretaceae spp insert ignore into journalissuearticles values( Combretum hartmannianum and Terminalia laxiflora );   analysis it by using chromatographic and spectroscopic analysis. Petroleum ether Nikhra fraction analysis with TLC and spray TLC with vanillin H 2 SO 4 insert ignore into journalissuearticles values(pink); insert ignore into journalissuearticles values(B1, B2);, Rf values insert ignore into journalissuearticles values(0.84, 0.81);, were expected to be phenolic, with vanillin HCL insert ignore into journalissuearticles values(red ); compounds spots insert ignore into journalissuearticles values(B2); with Rf values insert ignore into journalissuearticles values(0.81); was expected to be catechin and with vanillin H 3 PO 4 , blue-violet zones compounds spots insert ignore into journalissuearticles values(A1, A2, A3);, insert ignore into journalissuearticles values(B1, B2, B3, B4);, with Rf values insert ignore into journalissuearticles values(0.88, 0.78, 0.67);, insert ignore into journalissuearticles values(0.84, 0.81, 0.67, 0.59);, respectively were expected to be lignans. Petroleum ether fermented wood `Nikhra `   fraction was divided into two types of compounds classes aromatic and non aromatic by  and hence compounds were classified to phenolics and terpenoids compounds by GC/MS. Fragrant aromatics or terpenoids were targeted in this part of study. GC-MS analysis gave a spectrum of fragrance aromatic compounds insert ignore into journalissuearticles values(phenolics); in the petroleum ether Nikhra fractions of T. laxiflora, was Lup-20insert ignore into journalissuearticles values(29);-en-3-ol, acetate, insert ignore into journalissuearticles values(3β); and Tetracosamethyl-cyclododecasiloxane, main terponoids compounds was eicosamethylcyclodecasiloxane. Main fragrance aromatics compounds in the petroleum ether Nikhra fraction of C. hartmannianum was 2-tert-Butyl-5-insert ignore into journalissuearticles values(hydroxtmethyl);-4-formylfuran, and main terponoids compounds was Tetracosamethylcyclododecasiloxane.
Keywords : Combretaceae, Combretum hartmannianum, Terminalia laxiflora, Gas Chromatography