TLC and GC-MS analysis of petroleum ether fraction of fermented wood `Nikhra` of Acacia seyal

Authors : Abdalbasit MARIOD

Pages : 23-29

Doi:10.31195/ejejfs.472038

View : 12 | Download : 8

Publication Date : 2019-02-28

Article Type : Research Paper

Abstract :Aim of this study to detect organoleptically Acacia seyal fractions insert ignore into journalissuearticles values( petroleum ether, chloroform, methanol  and aqueous); fermented wood `Nikhra ` of the fractions accumulating the strongest sweet fragrance these fragrances were mainly accumulated in the petroleum ether fermented wood `Nikhra ` fraction and analysis it by using chromatographic and spectroscopic analysis. Petroleum ether fermented wood `Nikhra ` fraction analysis with TLC and spray TLC with vanillin H 2 SO 4 insert ignore into journalissuearticles values(pink); insert ignore into journalissuearticles values(C1, C2,C3,C4,C5,C6,C7);, Rf values insert ignore into journalissuearticles values(0.92, 0.86, 0.71, 0.64, 0.57, 0.50, 0.36); ,were expected to be phenolic, with vanillin HCL insert ignore into journalissuearticles values(red ); compounds spots insert ignore into journalissuearticles values(C5,C6,C7); with Rf values insert ignore into journalissuearticles values(0.57, 0.50, 0.37); was expected to be catechin and with vanillin H 3 PO 4 , blue-violet zones compounds spots insert ignore into journalissuearticles values(C6, C7);, with Rf values insert ignore into journalissuearticles values( 0.50, 0.36);, were expected to be lignans. Petroleum ether fermented wood `Nikhra ` fraction was divided into two types of compounds classes aromatic and non aromatic by http://research.easybib.com and hence compounds were classified to phenolics and terpenoids compounds by GC/MS. Fragrant aromatics or terpenoids were targeted in this part of study. GC-MS analysis gave a spectrum of fragrance aromatic compounds insert ignore into journalissuearticles values(phenolics); in the petroleum ether fermented wood `Nikhra ` fractions of A.seyal , was Petadecanoic acidinsert ignore into journalissuearticles values(5.64%); and Tetracosamethyl-cyclododecasiloxaneinsert ignore into journalissuearticles values(4.17%); total fragrance aromatic compoundsinsert ignore into journalissuearticles values(44.57%);, and main terponoids compounds was Octadecanoic acidinsert ignore into journalissuearticles values(2.52); % total fragrance aromatic compoundsinsert ignore into journalissuearticles values(11.87%);.
Keywords : Acacia seyal, Gas Chromatography GC,