THE DETERMINATION OF AMMELINE BY DIFFERENTIAL PULSE POLAROGRAPHY /APPLICATION TO MILK

Authors : Ummihan TASKOPARAN YİLMAZ, Zehra Yazar AYDIN

Pages : 549-558

View : 20 | Download : 7

Publication Date : 2016-09-30

Article Type : Research Paper

Abstract :A electrochemical approach has been proposed for the determination of ammeline. Electrocemical behavior of ammeline was investigated by differential pulse polarography and cyclic voltammetry in a Britton–Robinson buffer. Optimum conditions for the analytical determination were found to be pH 9.5 at a reduction potential of –30 mV. The calibration graph was linear in the concentration range of ammeline from 0,5 μM - 39,65 μM insert ignore into journalissuearticles values(0.06 µg mL -1 – 4.76 µg mL -1 ); with a correlation coefficient of 0.998. Based on this use and under optimized conditions, with the DPP method for ammeline analysis had detection of limit insert ignore into journalissuearticles values(LOD); and limit of quantification insert ignore into journalissuearticles values(LOQ); were obtained as 0.15 µM and 0.5 µM insert ignore into journalissuearticles values(0.02 µg mL -1 - 0.06 µg mL -1 );, respectively. This offered method has been used for the determination of ammeline in spiked milk. The result demonstrated that this method is a simple, rapid, sensitive, stable and low-cost method for ammeline detection.  
Keywords : ammeline, determination, polarography, milk, cyclic voltammetry