Immobilization of GOx on Trp / Trp-Fc Functionalized Nanospheres: Improved of Reusability and Stability

Authors : Nurdan KURNAZ YETİM, Elvan HASANOĞLU ÖZKAN, Nurşen SARI

Pages : 114-122

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Publication Date : 2017-12-11

Article Type : Research Paper

Abstract :In the study, new nanospheres support containing ferrocene were synthesized and characterized, then the properties of nanospheres as biocatalysis were researched . Novel support was prepared from insert ignore into journalissuearticles values(Aminomethyl);polystyrene insert ignore into journalissuearticles values(AMP);, tryptophan insert ignore into journalissuearticles values(Trp); and ferrocene aldehyde insert ignore into journalissuearticles values(Fc);  and the enzymatic properties of immobilized  glucose oxidase enzyme insert ignore into journalissuearticles values(GOx); was investigated. Modified polystyrene was characterized IR spectra, Gel permeation chromatography and Scanning electron microscopy. Ferrocene-tagged nanospheres-GOx shows high reusability and storage capacity and fast incubation time determination of glucose. After 12 months, immobilized GOx to the AMP-Trp and AMP-Trp-Fc retained ca 20 % and 40 % for their original activity, respectively. High activities were found for modified polymers AMP-Trp-Fc retains more than 35 % of the initial activity after fifteenth successive cycles, which is a perfect performance.
Keywords : Biocatalysis, ferrocene, glucose oxidase, nanospheres