Preparation of Surface Imprinted Magnetic Nanoparticles for Selective Detection of Ciprofloxacin in Milk

Authors : Adem ZENGİN

Pages : 72-85

View : 20 | Download : 13

Publication Date : 2017-12-11

Article Type : Research Paper

Abstract :Molecular imprinted polymers were synthesized for selective detection of ciprofloxacin on magnetic nanoparticles via surface initiated free radical polymerization. Methacrylic acid, ethylene glycol dimethacrylate, azobisisobutyronitrile and ciprofloxacin were used for the formation of molecular imprinted layer as a functional monomer, cross linker, initiator and template molecule, respectively. The surface characterization of the prepared nanoparticles were carried out by using several surface characterization methods such as TEM, XPS and VSM. The prepared imprinted nanoparticles had high binding capacity and fast adsorption kinetics. Additionally, the prepared nanoparticles showed superparamagnetic property with rapid magnetic separation and retained binding selectivity after ten adsorption-desorption cycles. The imprinted magnetic nanoparticles were subsequently applied for selective separation and determination of CPX from milk with high recoveries insert ignore into journalissuearticles values(98.0% - 98.8%); and low relative standard deviations insert ignore into journalissuearticles values(2.76% - 4.59%);. All the results have shown that the developed new method is a good alternative for the selective separation of ciprofloxacin in complex medium.
Keywords : magnetic nanoparticles, molecular imprinting, magnetic separation, ciprofloxacin, milk