THE EFFECTS OF 20-HYDROXYECDYSONE ON HEMOCYTES OF GALLERIA MELLONELLA (LEPIDOPTERA) IN VITRO CONDITIONS

ABSTRACT The effects of different concentrations of 20-hydroxyecdysone insert ignore into journalissuearticles values(20-E); on hemocytes of Galleria mellonella were investigated in vitro. Hemocyte culture was performed in Grace`s insect medium with fetal calf serum. The hormone was added to the cultures at two different concentrations. During a period of three days, the cells insert ignore into journalissuearticles values(plasmatocytes, granulocytes and total cell number); were counted under an inverted microscope. Obtained numbers were evaluated with one way variance analysis insert ignore into journalissuearticles values(ANOVA);. Hormone treatment led to a dose-dependent decrease in total cell and granulocyte number. The number of plasmatocytes, which are the predominant cells for phagocytose activity in immune response, was enhanced by hormone treatment.   Key Words: 20-hydroxyecdysone, hemocyte, in vitro, Galleria mellonella, insect