Spectrofluorimetric Determination of Diiodohydroxyquinoline in Presence of Metronidazole in Pharmaceutical Formulation and Spiked Human Plasma

Authors : Y ABOULENEIN, Nouruddin ALI, Mohammed GAMAL, Mohammed ABDELKAWY

Pages : 141-149

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Publication Date : 2013-07-05

Article Type : Research Paper

Abstract :Accurate and sensitive spectrofluorimetric method was developed for determination of diiodohydroxyquinoline in presence of metronidazole. In this method the native fluorescence of diiodohydroxyquinoline in water solvent at λ =495 nm when excitation was at 250 nm is used for its determination. Linear correlation was obtained in theconcentration range of 400 to 900 ng mL-1. The proposed method was successfully applied for determination ofdiiodohydroxyquinoline in bulk powder with mean accuracy of 100.21±1.13 or in spiked human plasma with mean accuracy of 100.53±1.42 without interference of metronidazole.
Keywords : spectrofluorimetry, Diiodohydroxyquinoline, Metronidazole