Fluorimetric Investigation of Host-Guest Complexation of Nizatidine with β-Cyclodextrin and its Analytical Application

Authors : Y ABOULENEIN, Abdalla ELBASHIR, Mohammed IBRAHIM

Pages : 151-156

View : 19 | Download : 15

Publication Date : 2013-07-05

Article Type : Research Paper

Abstract :The supramolecular interaction of nizatidine insert ignore into journalissuearticles values(NIZ); and β-cyclodextrin insert ignore into journalissuearticles values(β-CD); has been studied by spectrofluorimetry. The results showed that β-CD reacted with NIZ to form an inclusion complex.The NIZ-β-CDcomplex formed a host-guest complex in 1:1 stoichiometryand inclusion constant insert ignore into journalissuearticles values(K = 3.15×102 L mol-1); and wasascertained by the typical double reciprocal plots. Furthermore, the thermodynamic parameters insert ignore into journalissuearticles values(ΔH°, ΔS° and ΔG°);associated with the inclusion process were also determined. Based on the significant enhancement of the  fluorescence intensity of NIZ produced through complex formation, a simple, accurate, rapid and highly sensitive  spectrofluorometric method for the determination of NIZ in aqueous solution in the presence of β-CD wasdeveloped. The measurement of relative fluorescence intensity was carried with excitation at 314 nm.The factors affecting the inclusion complex formation were studied and optimized. Under the optimum reactionconditions, linear relationships with good correlation coefficients insert ignore into journalissuearticles values(0.999); were in the concentration range of 0.04-0.1μg mL–1for spectrofluorimetry. The limit of detection insert ignore into journalissuearticles values(LOD); was found to be1.2710-3 μgmL-1.The proposedmethod was successfully applied to the analysis of NIZ pharmaceutical dosage formulations with good accuracy; therecovery percentages ranged from 95.4–106.85%.
Keywords : Nizatidine, inclusion complex, β cyclodextrin, Spectrophotometry, Spectrofluorimetry