A New Laccase-Based Biosensor for Epinephrine Determination

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Gazi University Journal of Science
Volume:28 Issue:1
A New Laccase-Based Biosensor for Epinephrine Determination

A New Laccase-Based Biosensor for Epinephrine Determination

Authors : Fatma ARSLAN, Selma DURMUŞ, Özlem ÇOLAK, Halit ARSLAN

Pages : 1-9

View : 30 | Download : 15

Publication Date : 2015-02-23

Article Type : Research Paper

Abstract :In this paper, a novel amperometric e pinephrine insert ignore into journalissuearticles values(adrenaline); biosensor with immobilization of laccase on polypyrrole–polyvinylsulphonate insert ignore into journalissuearticles values(PPy-PVS); film has been a complished. Laccase enzyme were immobilized on PPy-PVS film by cross-linking with glutaraldehyde. Determination of EP was carried out by the reduction of enzymatically produced epinephrinequinone at - 0.220 V vs. Ag/AgCl. The effects of pH and temperature were investigated. There are two linear parts in the region between 0.1 µM-1.0 µM and 1.0 µM-10.0 µM. The storage stability and operation stability of the enzyme electrode were also studied. Interference effects were investigated on response of the biosensor.
Keywords : Epinephrine, laccase, biosensor, polypyrrole PPy, polyvinylsulphonate PVS,

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