Effects of Different Culture Media Compositions on In Vitro Micropropagation from Paradox Walnut Rootstock Nodes

Authors : Cem DİRLİK, Hacer KANDEMİR, Nurberat ÇETİN, Senem ŞEN, Begüm GÜLER, Aynur GÜREL

Pages : 500-515

Doi:10.54287/gujsa.1194822

View : 56 | Download : 12

Publication Date : 2022-12-31

Article Type : Research Paper

Abstract :The aim of this study is to establish an effective protocol for in vitro micropropagation from node explants of the \`Paradox\` insert ignore into journalissuearticles values(Juglans regia x J. hindsii); rootstock. In the first stage of the study, the node explants were cultured in semi-solid media after being exposed to different sterilization methods. The most effective sterilization method was observed as ST2 with the lowest darkening rate insert ignore into journalissuearticles values(4 %);. According to the results, SP4 medium provided the highest number of shoots per unit explant insert ignore into journalissuearticles values(1.05 shoots/explant); and 100 % shoot formation, SP2 medium provided the best mean shoot length insert ignore into journalissuearticles values(1.6 cm); and the mean number of nodes per unit explant insert ignore into journalissuearticles values(7.95 nodes/explant);. Best root induction was provided from ½ Modified MS medium containing 4 mg/L IBA with 30 g/L sucrose. For root induction explants were retained under dark conditions for 7 days at 24±2oC. In order to ensure root formation and elongation, shoot explants with root induction cultured in a 16-hour photoperiod insert ignore into journalissuearticles values(3500 lux); for 21 days in different nutrient media containing 2.4 mg/L Gelrite, hormone-free and mixed with vermiculite insert ignore into journalissuearticles values(1:1 v/v); and was rooting rate is 45.45 % in ¼DFe medium.
Keywords : Micropropagation, Walnut, Juglans regia L, Paradox, In vitro

ORIGINAL ARTICLE URL