Simultaneous Determination of Three Phenolic Compounds in Water Samples by Pre-column Derivatization Coupled with Reversed-Phase High Performance Liquid Chromatography

Authors : Adem ASAN, Mumut KONANÇ, Bedia AKMEŞE

Pages : 147-157

View : 24 | Download : 30

Publication Date : 2018-06-03

Article Type : Research Paper

Abstract :I n this study, a sensitive and accurate method for simultaneous separation and determination of three phenolic compounds insert ignore into journalissuearticles values(phenol, m-cresol and resorcinol); in water by revesed-phase high performance liquid chromatography using uv-visible detection has been described. Pre-column derivatization with 4-aminoantipyrine is used for the seperation and determination phenol, m-cresol and resorcinol in water. The derivatives formed within 5 min were extracted with chloroform and then analyzed by liquid chromatography with UV-visible detection at 440 nm. Chromatographic separation was performed using a reversed-phase column and acetonitrile-water insert ignore into journalissuearticles values(45:55%, v/v); as the mobile phase. The three derivatives were elueted in 13 min. The detection limits of phenol, m-cresol and resorcinol in a standard water sample were between 0.07 and 0.09 μg.L-1 for 100 mL respectively. The recoveries of the derivatives from pure water were betweeen 97.1-102.3% within relative standard deviations of 2.3-4.7%. The method was applied to the analysis of phenols in different water samples.
Keywords : Phenols, derivatization, reversed phase liquid chromatography, 4 aminoantipyrine