Synthesis and Characterisation of a New Hydrophilic Interaction/Reversed Phase Mixed-Mode Chromatographic Stationary Phase

Authors : Hayriye ARAL

Pages : 43-52

View : 22 | Download : 10

Publication Date : 2018-03-01

Article Type : Research Paper

Abstract :A novel multifunctional stationary phase based the amide-silica was synthesised starting from L-isoleucine and 2-aminoacetanilide. Hereby, compounds 1, 2, 3 and 4 were synthesised for the first time and 1, 2 and 4 were identified. The stationary phase was synthesised by a reliable and repeatable method and characterized by elemental analysis, solid state 13C NMR, scanning electron microscope insert ignore into journalissuearticles values(SEM);, and Brunauer, Emmett and Teller insert ignore into journalissuearticles values(BET);. This stationary phase possess four amide groups as polar cites, phenyl ring as aromatic non-polar cite and isopropyl as an aliphatic non-polar cite. Therefore, it can act as both hydrophilic interaction insert ignore into journalissuearticles values(HILIC); and reversed phase insert ignore into journalissuearticles values(RPLC); chromatographic stationary phase. The stationary phase has also chirality and can be used in enantioseparation of racemic compounds in normal phase chromatography insert ignore into journalissuearticles values(NPLC);.
Keywords : Mixed mode stationary phase, Hydrophilic interaction liquid chromatography HILIC, Isoleucine, Chiral stationary phase