Astragalus Trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate

Authors : Pınar NARTOP, Aynur GÜREL, İsmail Hakkı AKGÜN, Erdal BEDİR

Pages : 185-192

Doi:10.15671/hjbc.623783

View : 20 | Download : 8

Publication Date : 2019-09-18

Article Type : Research Paper

Abstract :I n this study, two grams of callus regenerated from stem and leaf explants of Astragalus trojanus Stev. were cultured in Woody Plant Medium insert ignore into journalissuearticles values(WPM); supplemented with 1 mg/L 2,4-D for four weeks and used as inoculum in order to investigate the effects of working volume and media composition. The highest biomass was obtained in 250 mL flask with astragaloside IV insert ignore into journalissuearticles values(1.66 µg/mg); and cycloastragenol insert ignore into journalissuearticles values(0.19 µg/mg); accumulation. Different concentrations of sucrose and casein hydrolysate insert ignore into journalissuearticles values(1 and 2 g/L); were also tested and the effect of pH was also investigated. Biomass accumulation cannot be enhanced, however, astragaloside IV and cycloastragenol content was ascended. The highest astragaloside IV insert ignore into journalissuearticles values(95.23 µg/mg); and cycloastragenol insert ignore into journalissuearticles values(5.93 mg/mg); accumulations were obtained at pH 6.8 and 2 g/L casein hydrolysate, respectively
Keywords : Astragalus trojanus, kesikli kültür, astragalozit IV, sikloastragenol