The Bioinformatic and Expression Analysis of PSMD4 Gene

Authors : Sümeyye AYDOĞAN TÜRKOĞLU, Gizem GÜLER, Feray KÖÇKAR

Pages : 329-336

View : 33 | Download : 11

Publication Date : 2018-09-01

Article Type : Research Paper

Abstract :26S proteasome non-ATPase subunit 4 insert ignore into journalissuearticles values(PSMD4); that has a molecular weight of 41 kDa is included on chromosome 1 insert ignore into journalissuearticles values(1q21.3);. PSMD4 protein is a subunit of the 19S regulatory region in the 26S proteasome. The proteasome breaks down proteins that are not needed or non-functional in the cell. We have limited data about the regulation of this gene in the literature. In our research, changes in the mRNA and protein levels of the PSMD4 gene were investigated in different cancer cell lines insert ignore into journalissuearticles values(prostate, breast, colon, cervix, hepatoma, osteosarcoma and pancreas); and a normal cell insert ignore into journalissuearticles values(human vein endothelial cell);. The highest expression of PSMD4 gene was seen in HUVEC and PC-3 cells. Bioinformatic analysis was also performed on PSMD4 protein for different species namely human, mouse and rat. Our Bioinformatic analyses showed that first 250 nucleotides are much conserved in all three species.
Keywords : PSMD4, cancer, cell lines, bioinformatics