In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A Mouse Connective Tissue Fibroblast Cells

Authors : Esin AKBAY, Y Doruk ARACAGÖK, Ali Hakan GÖKER, Mehmet Ali ONUR, Nilüfer CİHANGİR

Pages : 25-36

Doi:10.15671/hjbc.680564

View : 108 | Download : 7

Publication Date : 2021-01-01

Article Type : Research Paper

Abstract :The aim of this in vitro study was assessment of the cytotoxic level of 6-O-desmethylnaproxen. A mouse connective tissue fibroblast cell line, L929 was exposed to naproxen and 6-O-desmethylnaproxen in different concentrations for 24- and 48- hours. Cell viability was tested by MTT, while apoptosis was determined by AO/PI double staining method. The control group was free from any agent insert ignore into journalissuearticles values(DMEM only); and accepted as 100% cell viability. The IC50 results indicated that the inhibition of 50% cell viability was resulted by the highest concentration of 6-O-desmethylnaproxen insert ignore into journalissuearticles values(0.7 µg/mL); while none of naproxen concentration was caused 50% cell dead. Consequently, to avoid the unacceptable side effects of naproxen metabolites that evacuated by urine; further studies should be conducted to determine the accumulation of naproxen metabolites.
Keywords : Cytotoxicity, naproxen, 6 O desmethylnaproxen, in vitro

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