Development and Validation of a Quantification Method for L-DOPA in Plants and Pharmaceutical Materials

Authors : İbrahim BULDUK, Nurdoğan TOPAL

Pages : 1-10

Doi:10.15671/hjbc.773988

View : 83 | Download : 25

Publication Date : 2021-01-01

Article Type : Research Paper

Abstract :L-DOPA is an antiparkinsonian drug and is used per oral application. A reliable liquid chromatographic method for determination of L-DOPA in leaves, flowers and seeds of Vicia faba L. grown in Turkish habitats and pharmaceutical dosage forms has been developed. The analysis has been carried out using Ace C18 insert ignore into journalissuearticles values(5 µm, 4.6 x 250 mm,); column, and the separation was carried out using a mobile phase consisting of 50 mM potassium dihydrogen phosphate insert ignore into journalissuearticles values(pH 2.3); at a flow rate of 1.2 mL min-1 with UV detection at 280 nm. The method has been validated according to the acceptance criteria of the ICH guidelines. The method demonstrated good linearity insert ignore into journalissuearticles values(R2 > 0.999); over the assayed concentration range and showed good intra-day and inter-day precision. The detection limit insert ignore into journalissuearticles values(LOD); and the quantification limit insert ignore into journalissuearticles values(LOQ); values were determined as 1.70 and 5.13 μg mL-1 for L-DOPA. This method has been successfully used for the determination of L-DOPA in pharmaceutical formulations and plant materials.
Keywords : L DOPA, HPLC UV, METHOD, DEVELOPMENT, PLANT MATERIAL

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