Investigation of Cryptosporidium parvum and Giardia intestinalis in Various Water Sources in Isparta Area, Turkey

Authors : Abbas Yousefi RAD, Sevim AYSAL, Müge TANER

Pages : 209-217

View : 15 | Download : 7

Publication Date : 2007-08-01

Article Type : Research Paper

Abstract :Cryptosporidium parvum C. parvum and Giardia intestinalis G. intestinalis were investigated in samples collectedfrom lake, stream and drinking fountain within borders of Isparta city in Turkey. This study was conducted betweenJune and October 2004 and 40 water samples were collected. Seven water samples were collected from Eğirdir Lake,six from Gölcük Lake, ten from Darı Stream of Isparta flowing into Karacaören Dam after uniting Aksu Stream.Seventeen samples from fountains were collected from different regions of the city. These samples were filtered usinga membrane filtration system. After centrifuging, the filtered samples were investigated with direct microscopy forGiardia and with Modified Zielh-Nellsen MZN for Cryptosporidium. Existence of C.parvum was verified byimmunoflourescence assay IFA method. After the direct microscopy and MZN, C. parvum existence susficied in 13 32,5% of the 40 samples, and six of them 15% was confirmed by IFA method. Giardia cysts were found in 8 20% sample after direct microscopy
Keywords : Giardia intestinalis, Cryptosporidium parvum, drinking water, immunoflourescence assay