Nutritional Factors Effecting Rhamnolipid Production by a Nosocomial Pseudomonas aeruginosa

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Hacettepe Journal of Biology and Chemistry
Volume:36 Issue:1
Nutritional Factors Effecting Rhamnolipid Production by a Nosocomial Pseudomonas aeruginosa

Nutritional Factors Effecting Rhamnolipid Production by a Nosocomial Pseudomonas aeruginosa

Authors : Tugba SUBASİOGLU, Emir CANSUNAR

Pages : 77-81

View : 34 | Download : 16

Publication Date : 2008-01-01

Article Type : Research Paper

Abstract :Different Pseudomonas strains were isolated and grown on the basal medium and inspected for rhamnolipid biosurfactant production. They were incubated in a rotary shaker for 8 days at 30 oC and at 150 rev min-1. In order to increase the rhamnolipid production, 7 different carbon sources, including glucose, glycerol, mannitol, hexadecane, fructose, maltose and lactose were used. Varying concentrations of carbon sources for maximum rhamnolipid concentration were also determined. Different nitrogen sources in the basal medium, such as NH4 2SO4, NH4H2PO4, NH4NO3, KNO3, NaNO3 and peptone were tested. Resulting rhamnolipid concentration was 589.3 mg/L, when 2% mannitol was used as the carbon source and 2.0 g/L NaNO3 as the nitrogen source.
Keywords : Pseudomonas aeruginosa, rhamnolipid, biosurfactant, nosocomial bacteria

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