Removal of Butylated Hydroxyanisole with Enzyme Based Polymerization Using Organo-Clays

3,5-di-tert-butyl-4-hydroxytoluene BHT and 2-tert-butyl-4-methoxyphenol BHA are used as antioxidants in food. However, considerable concern has arisen from experiments showing that BHA and BHT are potent enhancers of chemically-induced mutagenesis and carcinogenesis in laboratory animals. This work describes a method for the removalof buytlated hydroxyanisole BHA compound with an enzyme based polymerization onto organo-clay from aqueoussolution. BHA bound organo-clay was prepared from horseradish peroxidase HRP -catalyzed polymerization reaction onto nanolayer of organosimectite. The approach described the enzyme catalyzed oxidation of BHA to quartamin modified smectite