A Kinetic Model Development for Phenol Removal via Enzymatic Polymerization

Authors : Mihriban ŞENGÖR, Nahit AKTAŞ

Pages : 295-301

View : 14 | Download : 9

Publication Date : 2009-12-01

Article Type : Research Paper

Abstract :Enzymatic oxidative polymerization of phenol catalyzed by laccase enzyme extracted from Trametes versicolor ATCC 200801 in an aqueous solution containing acetone was investigated in a batch system. The effects of initial phenol and dissolved oxygen concentrations on the initial reaction rate of oxidative polymerization were experimented. An interactive kinetic model as a function of phenol and dissolved oxygen concentrations was developed for enzymatic polymerization and corresponding bio-kinetic parameters have been evaluated through a non-linear regression program so called SYSTAT 10.0 trial version. The bio-kinetic coefficient of the developed model, which are Vmax, Kmm, KmO2 and k2 were obtained as 0.029 mg DO /L.min, 66.58 mg/L, 0.89 mg/L, and 254 s-1 respectively. The activation energy of oxidative phenol polymerization was calculated as 21.175 kJ/mol.
Keywords : laccase, Phenol, Activation energy, Enzymatic polymerization, Wastewater treatment