Purification and Characterization of Van Lake Fish Chalcalburnus tarichii P.1811 Liver and Brain Acethylcholinesterase

Authors : Esrin AKMAN, Vedat TÜRKOĞLU, Ismail CELİK

Pages : 331-336

View : 21 | Download : 5

Publication Date : 2009-12-01

Article Type : Research Paper

Abstract :In this study, acethylcholinesterase was purified from liver and brain of Van Lake fish Chalcalburnus Tarichii P.1811 by using affinity chromatography technique. The purification folds were determined as 142 and 344 for brain and liver, respectively. The purity of enzyme was controlled with sodium dodecylsulfate-polyacrylamide gel electrophoresis SDSPAGE . The optimal pH was determined as 7.5-8.5 for brain and liver, respectively. On the other hand, the optimal temperature was determined as 35oC for both liver and brain. Also, the highest activities were observed in concentration of 0.2 M NH4 2SO4 as ionic strength for both liver and brain.
Keywords : purification, characterization, Acethylcholinesterase, Chalcalburnus Tarichii, Fish