DNAJC25 as a tumor suppressor candidate gene in breast cancer

Authors : Tolga ACUN

Pages : 305-311

Doi:10.15671/hjbc.725263

View : 25 | Download : 7

Publication Date : 2021-05-17

Article Type : Research Paper

Abstract :DNAJC25 gene is a member of the HSP40 insert ignore into journalissuearticles values(DNAJ); family, and it was suggested as a tumor suppressor gene in hepatocellular carcinoma. The aim of this study was to analyze the expression, genetic/epigenetic alterations, and prognostic value of the DNAJC25 gene in breast cancer. DNAJC25 transcript levels are upregulated in BT-20 and ZR-75-1 cell lines and downregulated in MDA-MB-231 cell line compared to the non-tumorigenic mammary epithelial cell line insert ignore into journalissuearticles values(MCF 10A); insert ignore into journalissuearticles values(P< 0.05);. According to UALCAN in-silico tool, clinical breast cancer samples show significantly reduced levels of DNAJC25 mRNA levels relative to the normal samples insert ignore into journalissuearticles values(P=1.47e-02);. The Kaplan–Meier plotter tool shows that high DNAJC25 expression is favorable for post-progression survival in breast cancer insert ignore into journalissuearticles values(P=0.0035);. Point mutations or copy number variations of DNAJC25 are uncommon in clinical breast cancer samples. Combined bisulfite restriction analysis insert ignore into journalissuearticles values(COBRA); results showed that DNAJC25 promoter is not methylated in breast cell lines. Promoter hypomethylation was also observed in normal and tumor clinical samples insert ignore into journalissuearticles values(Beta-value < 0.25);. In conclusion, DNAJC25 is suggested as a tumor suppressor candidate having limited biomarker potential in breast cancer. Functional studies are essential to reveal its role in breast carcinogenesis.
Keywords : DNAJC25, HSP40, biomarker, breast cancer