A practical and single-use ITO-PET based immunosensing platform for detection of Tumor Necrosis Factor-alpha biomarker

Authors : Burcu ÖZCAN
Pages : 201-214
Doi:10.15671/hjbc.1215813
View : 39 | Download : 10
Publication Date : 2023-04-01
Article Type : Research Paper
Abstract :This investigation displays a novel, practical indium tin oxide- polyethylene terephthalate insert ignore into journalissuearticles values(ITO-PET); based electrochemical biosensor for the Tumor Necrosis Factor-alpha insert ignore into journalissuearticles values(TNFα); biomarker determination. The ITO-PET electrode is a very advantageous preferred semiconductive electrode material. It has a lot of great features such as easy to prepare, cheapness, flexibility, stability. It also allows determining an analyte at very low concentrations and provides a great wide concentration range for analyte analysis. Electrochemical Impedance Spectroscopy insert ignore into journalissuearticles values(EIS); and Cyclic Voltammetry insert ignore into journalissuearticles values(CV); were used for the evaluation of biosensors, including the immobilization procedure, the investigation of the optimum conditions, and the characterization of biosensors. The immunosensor\`s electrode surface morphology during the immobilization process was observed using a Scanning Electron Microscope insert ignore into journalissuearticles values(SEM);. In addition, Impedance measurement at a single frequency was used to characterize anti-TNFα and TNFα interactions insert ignore into journalissuearticles values(SFI);. The clinical effectiveness of the developed biosensor was investigated by testing it with real human serum samples. Moreover, the fabricated immunosensor presents long shelf life, analysis of the antigen concentrations at picogram level insert ignore into journalissuearticles values(0.02 pg mL-1 -2.56 pg mL-1);, reproducibility, reusability insert ignore into journalissuearticles values(11 times); and high sensitivity.
Keywords : TNFα, ITO PET elektrot, elektrokimyasal impedans spektroskopisi, immunosensör

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