Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches

Authors : Elmas GÖKOĞLU, Buse AKLAR, Tugba TASKIN TOK

Pages : 297-307

Doi:10.15671/hjbc.1272350

View : 62 | Download : 76

Publication Date : 2023-07-16

Article Type : Research Paper

Abstract :The binding properties of insulin hormone to the potential antidiabetic coumarin derivative umbelliferone insert ignore into journalissuearticles values(7hydroxycoumarin, 7HC); was investigated by absorption, fluorescence quenching and molecular docking methods. The negative signs of thermodynamic parameters insert ignore into journalissuearticles values(H and S); indicated that hydrogen bonds and van der Waals forces were dominant in the binding mode. The effect of common metal ions was investigated on binding parameters. According to the Förster’s theory; binding distance, r was obtained as 4.17 nm. The spectral data further supported by molecular docking calculations which show hydrogen bonds between 7HC and insulin.
Keywords : Insulin, fluorescence quenching, thermodynamic parameter, FRET