An Investigation upon DNA Interaction of 2 3 , 9 10 , 16 17 , 23 24 –Tetrakis 4- 4- 2-phenylprop-2-yl phenoxy Phthalocyanine Compound

Authors : Ali ARSLANTAS, Mehmet Salih AGİRTAS, Cihan DURMUS

Pages : 81-87

Doi:10.17350/HJSE19030000176

View : 26 | Download : 15

Publication Date : 2020-06-26

Article Type : Research Paper

Abstract :The DNA binding activity of previously synthesized and characterized 2 3 , 9 10 , 16 17 , 23 24 –Tetrakis 4- 4- 2-phenylprop-2-yl phenoxy phthalocyanine compound PcF with CT-DNA was studied using UV/Vis, emission spectroscopic titrations, the melting temperature, viscosity measurement, and agarose gel electrophoresis methods in a Tris-HCl buffer solution at a pH of 7.1 at room temperature. The absorption titration spectra studies of PcF showed that absorbance intensities were decreased with increasing of concentrations of CT-DNA and the finding proved that the compound interacts with the DNA. Addition to absorption titration study, emission spectroscopic titration, the melting temperature, viscosity measurement, and agarose gel electrophoresis methods were also performed to investigate the binding activities of PcF with the DNA. The results of these methods confirmed the findings of absorption spectra study that the compound interacts with the DNA.
Keywords : Absorption spectra, Fluorescence spectroscopy, DNA binding, Phthalocyanines