Enhanced Enzyme Activity with Ferritin Nanocages

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Hittite Journal of Science and Engineering
Volume:5 Issue:2
Enhanced Enzyme Activity with Ferritin Nanocages

Enhanced Enzyme Activity with Ferritin Nanocages

Pages : 115-118

View : 16 | Download : 11

Publication Date : 2018-06-29

Article Type : Research Paper

Abstract :I n this study, a novel enzyme system based on ferritin nanocages was designed. α-Amylase was covalently crosslinked on surface of ferritin nanocages by EDC/NHS. The activity of immobilized enzyme was monitored by using UV-vis spectrophotometer. The optimum temperature of immobilized enzyme shifted from 50 oC to 70 oC due to the ferritin nanocages. In addition, The Km and Vmax values of immobilized enzymes were 5.19 mg mL-1 and 3.3x10-5 U mg-1, respectively. This novel enzyme system displayed higher catalytic activity and enhanced stability
Keywords : Ferritin nanocages, α amylase, Enzyme immobilization, EDC NHS, Covalently crosslinked

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