Poli(Rutin) Micro/nanogels for Biomedical Applications

Authors : Mehtap ŞAHİNER, Selin SAGBAS SUNER

Pages : 179-187

Doi:10.17350/HJSE19030000228

View : 18 | Download : 17

Publication Date : 2021-06-30

Article Type : Research Paper

Abstract :Flavonoids are natural phenolic compounds that are active molecules commonly found in woody and herbaceous plants and used in natural defense mechanisms of plants against harmful microorganisms. In this research, rutin insert ignore into journalissuearticles values(RUT); molecule which is a natural flavonoid was bounded with epoxy groups of polyinsert ignore into journalissuearticles values(ethylene glycol); diglycidyl ether crosslinker by using micro emulsion crosslinking technique to synthesize of polyinsert ignore into journalissuearticles values(rutin); insert ignore into journalissuearticles values(pinsert ignore into journalissuearticles values(RUT);); micro/nanogels. These pinsert ignore into journalissuearticles values(RUT); micro/nanogels showed spherical morphologic structure with 0.3 nm-2 µm dry size range due to scanning electron microscope image. Furthermore, pinsert ignore into journalissuearticles values(RUT); micro/nanogels was found to be in injectable size range with 548±8.9 nm average size distribution in aqueous solution depends on the dynamic light scattering insert ignore into journalissuearticles values(DLS); measurement. In addition, the zeta potential measurements performed at different pH conditions and potentiometric titration of the prepared pinsert ignore into journalissuearticles values(RUT); micro/nanogels was also determined and the isoelectronic point and pKa values of these micro/nanogels was estimated as pH 2.85 and 2.16, respectively. The effects of RUT and pinsert ignore into journalissuearticles values(RUT); micro/nanogels on α-glycosidase insert ignore into journalissuearticles values(AG); insert ignore into journalissuearticles values(EC 3.2.1.20); enzyme activity were investigated and it was found that RUT and pinsert ignore into journalissuearticles values(RUT); micro/nanogels stimulate this enzyme. Moreover, fibrinogen interaction results showed that pinsert ignore into journalissuearticles values(RUT); micro/nanogels were more compatible than RUT for vascular system with less interaction ability of fibrinogen of pinsert ignore into journalissuearticles values(RUT); micro/nanogels. In addition, pinsert ignore into journalissuearticles values(RUT); micro/nanogels observed highest antioxidant scavenging ability with 251.3±20.2 gallic acid equivalency total flavonoid content insert ignore into journalissuearticles values(TFC); at 250 µg/mL and reduced of 1.75±0.27 µmol Fe insert ignore into journalissuearticles values(III); even for 5 µg pinsert ignore into journalissuearticles values(RUT); microgel/nanogels. Furthermore, no Fe insert ignore into journalissuearticles values(II); chelating activity was obtained for RUT monomer, but pinsert ignore into journalissuearticles values(RUT); micro/nanogels showed significant Fe insert ignore into journalissuearticles values(II); chelating activity as 43.11±17.4 %.
Keywords : Rutin, microgel, nanogel, alpha glucosidase enzyme, antioxidant