Lysozyme Imprinted poly(hydroxyethyl methacrylate) Cryogel Membrane with the Assistance of polydopamine

Authors : Aykut Arif TOPÇU

Pages : 77-82

Doi:10.17350/HJSE19030000294

View : 17 | Download : 8

Publication Date : 2023-03-31

Article Type : Research Paper

Abstract :The surface imprinted technique was utilized in creating the synthetic lysozyme receptors on the polyinsert ignore into journalissuearticles values(hydroxyethyl methacrylate); [pinsert ignore into journalissuearticles values(HEMA);] cryogel membrane with the assistance of polydopamine under alkaline conditions. Following the design of the artificial receptors, Fourier-transform infrared spectroscopy insert ignore into journalissuearticles values(FTIR);, scanning electron microscope insert ignore into journalissuearticles values(SEM); and the swelling test were used for the characterization studies and the lysozyme adsorption capability of the adsorbent was evaluated in the aqueous solution. The maximum adsorption capacity insert ignore into journalissuearticles values(Qmax); of lysozyme was found as 108.9 mg/g and the adsorption of the target protein on the adsorbent was monolayer and showed a good fit to the Langmuir isotherm model. The IF factor was 3.26 and the adsorbent was highly selective against creatinine and bovine serum albumin insert ignore into journalissuearticles values(BSA);. Furthermore, the adsorbent maintained its stability after 5 adsorption, desorption, and regeneration cycles.
Keywords : lysozyme, adsorption, molecular imprinting, p HEMA, dopamine, cryogel