GIBBERELLIN APPLICATION INFLUENCES ON EX VITRO GROWTH, FLOWERING AND STEVIOL GLYCOSIDE ACCUMULATION OF STEVIA REBAUDIANA BERTONI

Authors : Catherine ARRADAZA, Ma Lourdes O CEDO, Rocelie R ZARA, Rachel Ann GONZAGA

Pages : 75-83

View : 71 | Download : 15

Publication Date : 2019-06-18

Article Type : Research Paper

Abstract :Stevia insert ignore into journalissuearticles values( Stevia rebaudiana  Bertoni);   is a small perennial herb with incredible sweetening property. Stevia is known to contain steviol glycosides insert ignore into journalissuearticles values(SGs);, which are about 300 times sweeter than sucrose at their concentration of 4% insert ignore into journalissuearticles values(w/v);. However, the growth of s tevia plants and their SGs production are known to be influenced by external and internal factors. In this study, an ex-vitro experiment was conducted to determine the effect of exogenous GA 3 application on the growth, flowering and steviol glycoside insert ignore into journalissuearticles values(stevioside and rebaudioside A); accumulation in stevia under Philippine condition. A p reviously established tissue culture-derived plant was used as mother plants and categorized into two groups: insert ignore into journalissuearticles values(1); non-flowering insert ignore into journalissuearticles values(NF); and insert ignore into journalissuearticles values(2); flowering insert ignore into journalissuearticles values(F);. Stevia stem cuttings were sprayed with GA 3 solutions of various concentrations: 0, 50, 100 and 200 mg L -1 at 1-week interval for 5 weeks. NF plants sprayed with GA 3 showed significant difference in shoot length, number of shoot tips, length of longest primary branches compared to the control. NF plants produced more leaves than F at any GA level but without significant difference. Application of gibberellic acid insert ignore into journalissuearticles values(GA 3 ); neither delayed nor inhibits flowering of ex vitro stevia plant but significantly reduced the flowering intensity of the plant. Exogenously applied GA 3 also did not affect SG accumulation in the leaves and flowers.
Keywords : Stevia rebaudiana Bertoni, steviol glycosides, gibberellin, steviol glycosides

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