Heterologous Expression and Molecular Cloning from Williamsia Marianensis

Authors : Alaa Kadhim Shareef SHAREEF, Faez WAHEED, Belgin ERDEM, Ahmed Jasim NEAMAH, Ahmed Sadeq Habeeb ALADBAN

Pages : 69-73

Doi:10.22399/ijcesen.1133001

View : 13 | Download : 8

Publication Date : 2022-11-30

Article Type : Research Paper

Abstract :The majority of therapy methods include downsides and limits. As a result, many researchers are focused on developing effective remedies. Therapeutic peptides, like proteins and antibodies, are a potential class of medications that have a number of advantages over traditional pharmaceuticals. Williamson marianensis-produced cholesterol oxidase has been demonstrated to have medicinal value. Using PCR and primers specific to an expression vector insert ignore into journalissuearticles values(pET28b);, we were able to clone the cholesterol oxidase gene and express it in E. coli insert ignore into journalissuearticles values(BL-21/DE3); Rosetta following identification with IPTG. Genscript Corporation in the United States sequenced gyncholestrol oxidase insert ignore into journalissuearticles values(500 bp); to create a cox sequence, which was then submitted for synthesis. pET 28ainsert ignore into journalissuearticles values(+); cox William showed a twofold restriction digestion pattern. The pattern was made up of two strands: one was a carrier plasmid insert ignore into journalissuearticles values(4200 bp); and the other was a 2800 base pair strand that contained the cholesterol oxidase gene. The cholesterol oxidase gene was successfully cloned and expressed as a consequence. Williamson marianensis-derived cholesterol oxidase will be exploited in future medicinal re
Keywords : Human infections, Williamsia, PCR