Wet cupping therapy removes oxidative stress related miRNAs

Authors : Berivan UNAT, Fatma Hümryra YERLİKAYA, Hayriye BALTAOĞLU ALP, Duygu ERYAVUZ ONMAZ

Pages : 178-185

Doi:10.47482/acmr.1285642

View : 34 | Download : 39

Publication Date : 2023-09-27

Article Type : Research Paper

Abstract :Background: Wet cupping therapy is commonly used in different conditions such as hypertension, diabetes, inflammatory and infectious diseases. The mechanism of action of wet cupping therapy is not yet clear, however several studies have demonstrated that it has a role in limiting oxidative stress. The aim of this study was to investigate the effect of wet cupping therapy on oxidative stress-related miRNAs miRNA-34a, miRNA-200a, miRNA-21 levels and oxidant-antioxidant status markers malondialdehyde insert ignore into journalissuearticles values(MDA); and glutathione insert ignore into journalissuearticles values(GSH); levels. Methods: 60 healthy volunteer women aged 20-75 years insert ignore into journalissuearticles values(n=30, 20-45 years; n=30, 45-75 years); who were applied wet cupping at Konya Necmettin Erbakan University Meram Medical Faculty Traditional and Complementary Medicine Center were included in this study. Plasma miRNA-34a, miRNA-200a and miRNA-21 levels were analyzed by using real-time quantitative PCR insert ignore into journalissuearticles values(RT-PCR); analysis. MDA and GSH levels were measured using the commercial ELISA kits. Results: MDA levels [2003.10 insert ignore into journalissuearticles values(1810.17-7392.15); vs 1884.04 insert ignore into journalissuearticles values(1800.10-4488.05);, p=0.027] of wet cupping blood samples higher than venous blood samples, while GSH levels [125.97 insert ignore into journalissuearticles values(90.65-219.43); vs 131.77 insert ignore into journalissuearticles values(88.77-246.42);, p=0.037] were lower. miRNA-34a [insert ignore into journalissuearticles values(0.94±0.74); vs insert ignore into journalissuearticles values(0.53±0.28);, p<0.001], miRNA-21 [0.058 insert ignore into journalissuearticles values(0.01-0.54); vs 0.033 insert ignore into journalissuearticles values(0.01±0.18);, p=0.001] and miRNA-200a [24.42 insert ignore into journalissuearticles values(7.46-103.97); vs 20.32 insert ignore into journalissuearticles values(4.32-56.49);, p=0.037] levels of wet cupping blood samples were significantly higher than venous blood samples. Conclusions: Wet cupping therapy eliminates oxidative stress-related miRNAs from the body. Therefore, it is seen as a promising method in the welfare of reactive oxygen species insert ignore into journalissuearticles values(ROS);-related diseases by limiting oxidative damage.
Keywords : Cupping theraphy, miRNAs, oxidative stress, reactive oxygen species