Investigation of the Exosome-Based Drug Delivery System Potential in theTreatment of Glioblastoma in vitro Experimental Models

Authors : Sıdıka GENÇ, Zeynep ÇAKIR, Ali TAGHİZADEHGHALEHJOUGHİ, Yeşim YENİ, Kiyumars JALİLİ, Ahmet HACIMÜFTÜOĞLU

Pages : 451-467

Doi:10.38001/ijlsb.990646

View : 28 | Download : 10

Publication Date : 2021-12-15

Article Type : Research Paper

Abstract :In our study, it was aimed to create a new drug delivery system by loading the chemotherapeutic drugs into exosome vesicles. This system act as a Trojan horse to targeting GBM cancer. Exosomes were isolated from the T-98G cell line and characterized. Exosomes were encapsulated with Temozolomide. Then, the effectiveness of this new delivery system was evaluated by using MTT, LDH, TAC, TOS, GR. MTT results show a dose-dependent decrease in the TMZ and Exolimer+TMZ groups. But this rate decreased significantly in the Exolimer+TMZ groups. The lowest viability was observed at the Exolimer+TMZ 800 ng/ml concentration. Our results in LDH, GR, TAS and TOS analyzes shows correlation with MTT. Based on this study, we think that targeting the exosome to cancer by combining with special molecules and nanotechnology will bring a new perspective to cancer treatment.
Keywords : Exosome, Glioblastoma, TEM, Temozolomide