Diversity Analysis of Common Vetch (Vicia Sativa L.) Lines and Cultivars Using Pairwise Combinations of Universal Rice Primers

Authors : Mustafa TOPU, İskender TİRYAKİ

Pages : 504-518

Doi:10.38001/ijlsb.1122987

View : 21 | Download : 11

Publication Date : 2022-12-15

Article Type : Research Paper

Abstract :This study has been conducted to determine genetic diversity of the common vetch lines and cultivars by using pairwise combinations of universal rice primers insert ignore into journalissuearticles values(URPs);. A total number of 37 URP marker pairs were tested and twenty of those provided amplicons in the common vetch genome. The pairs of amplified URP markers provided a total of 83 bands and 62 of them were determined as polymorphic and were scattered to the whole genome. The average polymorphism rate of the primers was calculated as 73.5% while the polymorphism information content insert ignore into journalissuearticles values(PIC); values have ranged from 0.11 to 0.47 with an average of 0.24. The phylogenetic tree constructed based on UPGMA analysis provided three main clades. Two-dimensional plot of PCA and the UPGMA analysis showed that the URP markers successfully distinguished the genetic material based on their genetic origin. In conclusion, this study revealed that the use of pairwise combinations of URP markers could have a better power to reveal the level of polymorphism in plant genome.
Keywords : Common vetch, genetic diversity, molecular characterization, URP