In vitro Antibacterial, Antioxidant and DNA Damage Protective Activity of Blackberry (Rubus fruticosus L.) Root Extracts

Authors : Hatice Aysun MERCİMEK TAKCI, Sema GENÇ, Ayşenur YALÇIN, Eda ÖZDEMİR

Pages : 225-234

Doi:10.38001/ijlsb.1085539

View : 26 | Download : 11

Publication Date : 2022-08-15

Article Type : Research Paper

Abstract :The aim of this study is to explore the antibacterial, antioxidant, and DNA damage protection potentials of blackberry insert ignore into journalissuearticles values(Rubus fruticosus L); root extracts. Antioxidant activity of root extracts was researched by using DPPH˙ free radical scavenging and reducing capacity analysis. Methanolic extract of blackberry root system showed the maximum activity for TPC, TFC, DPPH˙, and ferric reducing capacity. Antibacterial activity of blackberry root extracts was screened against clinic isolates insert ignore into journalissuearticles values(Escherichia coli, Klebsiella spp., Proteus spp., Pseudomonas spp. and Staphylococcus aureus); by the Kirby Bauer method. Although the metanolic extract possessed a significant antibacterial activity at 100 mg/mL concentration, distilled water extract did not show any inhibition to clinic isolates. The protective effects of root extracts on pBR322 plasmid DNA against the mutagenic effect of UV photolysis of H2O2 were tested. All concentrations of methanolic and distilled water extracts were observed to protect DNA damage in the presence of H2O2 and UV. These results indicated that the presence of antioxidant substances of root system extract of blackberry insert ignore into journalissuearticles values(Rubus fruticosus L); can be effective against harmful effects of free radicals
Keywords : Antioxidant activity, antibacterial activity, blackberry, DNA damage protective, root extract