Effect of Salt and pH Stress of Bioactive Metabolite Production in Geitlerinema carotinosum

Authors : Tunay KARAN, Ramazan ERENLER

Pages : 16-19

Doi:10.21448/ijsm.356247

View : 18 | Download : 10

Publication Date : 2017-11-25

Article Type : Other Papers

Abstract :Cyanobacterial metabolites are natural products that have an important feature in pharmaceutical and medicinal industries. In this study, the presence of the secondary metabolite norharmane in the indole structure was determined in Geitlerinema carotinosum insert ignore into journalissuearticles values(Geitler); Anagnostidis isolated from Tokat Yesilirmak River and its production in salt stress and pH stress was investigated. In salt stress experiments, cyanobacterium was cultured for two weeks by adding NaCl to BG11 medium in erlenmayers of 0.5, 1.0, 3.0, 5.0 M. pH stress was executed at 5 and 9. Norharmane amount was determined by HPLC using C18 reverse phase column at a temperature of 40 °C and a flow rate of 1 ml / min. The amount of norharman metabolite insert ignore into journalissuearticles values(μg/g); was calculated according to the Gauss method by drawing a calibration curve over the absorbance value of the standard 247 nm wavelength. According to the analysis results, metabolite production was 0.612, 1.299, 0.011 at 0.5 M, 1.0 M, 3.0 M respectively. At 5 M, there was no norharmane production. The norharmane production is higher at pH 5 insert ignore into journalissuearticles values(1.293 μg /g); than that of the pH 9 insert ignore into journalissuearticles values(0.448 μg /g);.
Keywords : Geitlerinema carotinosum, stress conditions, norharmane, HPLC