In Vitro Bulb Regeneration from Stem Explants of Endemic Geophyt Muscari aucheri (Boiss.) Baker

Authors : Fethi Ahmet Özdemir, Ömer Kılıç, Eyüp Bağcı

Pages : 50-54

Doi:10.21448/ijsm.356264

View : 26 | Download : 10

Publication Date : 2017-11-25

Article Type : Research Paper

Abstract :Muscari aucheri insert ignore into journalissuearticles values(Boiss.); Baker bears beautiful and attractive purplish blue flowers bloom between May and June; it grows stony slopes, mountain pastures an altitude of 1000-3000 meters. The natural populations of Muscari aucheri, is seriously affected by increased environmental pollution, urbanization and grazing. Therefore, this study aimed to accelerate multiplication by devising a strategy for an efficient in vitro bulblet regeneration system of Muscari aucheri using stem explants on MS medium containing 1, 3, 5 mg/l TDZ plus 0, 0.1, 0.2, 0.4 mg/l NAA insert ignore into journalissuearticles values(12 combinations);. The stem explants induced direct bulblet regeneration on explants. Maximum mean number of bulblets per explant was noted on MS medium containing 3 mg/l TDZ + 0.4 mg/l NAA and maximum mean number of bulb diameter was noted on MS medium containing 5 mg/l TDZ + 0.2 mg/l NAA. The regenerated bulblets were isolated from stem explants and cultured on MS medium containing 40 g/l sucrose; where they gained diameter and rooted
Keywords : Muscari aucheri, in vitro, bulb regeneration