Effect of Nitrogen, Phosphorus and Medium pH to Enhance Alkaloid Production from Catharanthus roseus Cell Suspension Culture

Authors : Malay Ranjan Mishra MİSHRA, Rajesh Kumar SRİVASTAVA, Nasim AKHTAR

Pages : 137-153

Doi:10.21448/ijsm.559679

View : 17 | Download : 14

Publication Date : 2019-07-15

Article Type : Research Paper

Abstract :Several elevated levels of nitrogen and phosphate at varying pH of the medium which impart a major influence on callus and biomass development and subsequent production of alkaloids was investigated using suspension culture system of Catharanthus roseus in the present study. The B5 medium was buffered at pH 4.51, 5.82 and 7.32 by addition of different levels of insert ignore into journalissuearticles values(A); diammonium hydrogen phosphate insert ignore into journalissuearticles values(NH4);2HPO4 and insert ignore into journalissuearticles values(B); ammonium dihydrogen orthophosphate insert ignore into journalissuearticles values(NH4H2PO4); representing the enhanced and varied supply of total nitrogen insert ignore into journalissuearticles values(NH4++NO3ˉ); and phosphate compared to MS medium insert ignore into journalissuearticles values(as control); for cell biomass production and alkaloid yield. The pH of the medium have shown significant effects with maximum biomass fresh wt., dry wt. and total alkaloid yield at 5.82 medium pH with elevated phosphate levels and total nitrogen concentration of 3710.10 mg/L compared to control MS medium with 2850 mg/L total nitrogen. At 3667.33 and 3752.48 mg/L of total nitrogen with enhanced phosphate supply showed reduced biomass fresh wt., dry wt. and total alkaloid yield at lower insert ignore into journalissuearticles values(4.51); and higher insert ignore into journalissuearticles values(7.32); medium pH respectively. Inclusion of 200 mg/L of tryptophan or phenylalanine as reduced nitrogen source in B5 medium buffered at 5.82 ± 0.2 pH showed enhanced biomass and alkaloid production. Hence, addition of nitrogen, phosphate, tryptophan, phenylalanine as nutrient in suspension culture stimulate their uptake to enhance cell biomass and total alkaloids production but as a function of pH of the medium.
Keywords : Alkaloid, Catharanthus roseus, medium pH, Nitrogen, Phenylalanine, Phosphorus, Tryptophan