Effects of Methyl Jasmonate and Putrescine on Tryptanthrin and Indirubin Production in in vitro Cultures of Isatis demiriziana Mısırdalı

Authors : Özgür KARAKAŞ

Pages : 241-250

Doi:10.21448/ijsm.521498

View : 40 | Download : 12

Publication Date : 2019-10-15

Article Type : Research Paper

Abstract :Tryptantrin and indirubin are pharmacologically active compounds used in treatment of disseases such as cancer and Alzheimer`s. In this study, we investigated the influences of different concentrations of methyl jasmonate insert ignore into journalissuearticles values(MeJa); and putrescine insert ignore into journalissuearticles values(Put); on tryptanthrin and indirubin production in leaf explants and development of Isatis demiriziana Mısırdalı grown in vitro. In all media treated with methyl jasmonate, tryptanthrin production in leaves of plantlets showed an increase. The highest increase in tryptanthrin production was observed in solid Murashige-Skoog insert ignore into journalissuearticles values(MS); medium containing 1.0 mM MeJa insert ignore into journalissuearticles values(154.026 ± 0.11 µg g-1);, about 2.85-fold higher than the control insert ignore into journalissuearticles values(untreated plantlets); insert ignore into journalissuearticles values(40.017 ± 0.031 µg g-1);. Production of tryptanthrin decreased about 2.56-fold in the leaves of plantlets treated with Put, when compared to control. The highest indirubin production was obtained in the leaves of plantlets grown in the MS medium containing 0.1 mM MeJa insert ignore into journalissuearticles values(11.274 ± 0.035 µg g-1); but treatments with Put didn’t show any positive affect on the indirubin production. Analysis of tryptanthrin and indirubin were performed using high performance liquid chromatography insert ignore into journalissuearticles values(HPLC);.
Keywords : Tryptanthrin, Indirubin, Methyl jasmonate, Putrescine, Isatis demiriziana

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