Chemical content profile and antioxidant activity of Rhododendron ponticum L. (Ericaceae) extracts

Authors : Yusuf Sıcak, İrfan Öztürk, Bihter Şahin, Dilaycan Çam, Cansel Cakır, Mehmet Öztürk

Pages : 507-513

Doi:10.21448/ijsm.1382522

View : 56 | Download : 74

Publication Date : 2024-08-29

Article Type : Research Paper

Abstract :Rhododendron species (Ericaceae) is considered the most diverse group, with more than 1200 species famous for their colorful flowers. Rhododendron, also known as azalea, is a flowering tree in the Ericaceae family. Herein, in vitro antioxidant activities of acetone and methanol extracts of Rhododendron ponticum leaves were studied along with their phenolic contents using High-Performance Liquid Chromatography with a Diode-Array Detector (HPLC-DAD). Antioxidant activity was performed spectrophotometrically using ABTS·+, DPPH·, CUPRAC, and β-carotene/linoleic acid assays. Acetone extract showed better antioxidant activity than methanol extract in all tests. The HPLC-DAD analysis revealed fifteen phenolic compounds, of which seven were common for both extracts. Catechin (25.80 and 33.08 mg/g extract, respectively) and epicatechin (31.15 and 26.54 mg/g extract, respectively) were calculated as major phenolic components in acetone and methanol extracts.
Keywords : Rhododendron ponticum, HPLC DAD, Phenolic compound, Antioxidant activity