IAD Index of Academic Documents
  • Home Page
  • About
    • About Izmir Academy Association
    • About IAD Index
    • IAD Team
    • IAD Logos and Links
    • Policies
    • Contact
  • Submit A Journal
  • Submit A Conference
  • Submit Paper/Book
    • Submit a Preprint
    • Submit a Book
  • Contact
  • International Journal of Traditional and Complementary Medicine Research
  • Volume:3 Issue:3
  • In Vitro Inhibitory Potential of Lawsonia inermis Extracts against Multidrug Resistant Clinically-Re...

In Vitro Inhibitory Potential of Lawsonia inermis Extracts against Multidrug Resistant Clinically-Relevant Bacteria: a Phytochemical, Quantitative Antimicrobial and Toxicological Assessment

Authors : Morufat OLAİTAN, Blessing NWADİKE
Pages : 167-183
Doi:10.53811/ijtcmr.1193807
View : 17 | Download : 12
Publication Date : 2022-12-15
Article Type : Research Paper
Abstract :Objective: Majority of the current antibiotics have become less effective due to widespread of multidrug-resistant microorganisms. Medicinal plants are promising candidates that could be used to manage this menace. Therefore, phytochemical, toxicological and antimicrobial potentiality of Lawsonia inermis extracts against MDR clinical bacteria were carried out. Material-Method: Henna leaf and seed were extracted by cold maceration technique using methanol and water and screened phytochemically. Eight MDR isolates, four of which are ESβL-producers were used for this study. In vitro antimicrobial efficacy and quantitative antimicrobial potency of extracts were estimated. MIC and MBC were determined using broth macrodilution technique. Cytotoxicity test was conducted using brine shrimp lethality assay and LC50 was determined. Results: The findings of this study revealed that aqueous leaf extract possesses maximum percentage yield of 25.58%. Tannins and phenolic compounds were detected in all extracts, while steroid was absent. Methanol seed extract showed the highest antimicrobial efficacy against all bacteria with 100 percent activity. The highest and lowest zones of inhibition were recorded at 30.0±0.00 and 10.0±0.00 mm, respectively. The zones of inhibition of extracts differed significantly. All extracts displayed highest activity index against the ESβL-producing Enterobacter aerogenes 196 that was isolated from wound with highest value at 4.28. Pseudomonas aeruginosa U109 showed maximum susceptibility index insert ignore into journalissuearticles values(93.75%);; majority of MIC values recorded were within the range of 1.95-62.5 mg/mL. Cytotoxicity test of methanol and aqueous extracts displayed 1000 1000, respectively. Conclusion: Findings from this study elucidate the efficacy of Lawsonia inermis as a potential remedy to manage MDR-related infectious bacteria.
Keywords : Antimicrobial Resistance, Multidrug Resistant Microorganisms, ESBL, Antimicrobial Activity, Indigenous Plant, Phytotherapy

ORIGINAL ARTICLE URL
VIEW PAPER (PDF)

* There may have been changes in the journal, article,conference, book, preprint etc. informations. Therefore, it would be appropriate to follow the information on the official page of the source. The information here is shared for informational purposes. IAD is not responsible for incorrect or missing information.


Index of Academic Documents
İzmir Academy Association
CopyRight © 2023-2025