Estimation of total tannin and total phenolic content in plant (Crataegus azarolus L) by orbital shaker technique

Authors : Holem Hashm BALAKY, Karzan KHALİD, Avan HASAN, Shadya TAHİR, Sedat UBUR, Ali KHEDİR

Pages : 1-6

Doi:10.31015/jaefs.2021.1.1

View : 11 | Download : 12

Publication Date : 2021-03-31

Article Type : Research Paper

Abstract :The plant of Crataegus Azarolus L, genus that belongs to the Rosaceae family and is a low, dense, spiny tree with a beautiful inflorescence, the phytochemical investigations on genus Crataegus were mainly performed on the leaves, flowers and berries. The objective of this experiment was to determine the ratio of tannin and phenol compound by method orbital shaker extraction. The plant of Crataegus azarolus L was collected in hasanbag mountain soran, Kurdistan. The parts of seed, leaf and steam of Crataegus Azarolus L was extract with water solvent and orbital shaker and assessed yield extraction, total tannin and total phenol. The highest significant value insert ignore into journalissuearticles values(p<0.05); of yield extraction was observed from leaf insert ignore into journalissuearticles values(71.56%);, compared with other part of Crataegus Azarolus L. On the other hand, the total tannin was showed uppermost in leaf insert ignore into journalissuearticles values(3.03 kg/mg);, and the steam insert ignore into journalissuearticles values(2.87 kg/mg);. Whereas, the smallest total tannin was observed from the seed insert ignore into journalissuearticles values(1.10%);. Furthermore, the total phenolic contents of the plant were observed the lowest value in seed as insert ignore into journalissuearticles values(0.98 mg GAE/g);. Whereas, the total phenol in leaf was observed a higher significantly value insert ignore into journalissuearticles values(3.68 mg GAE/g); and in steam insert ignore into journalissuearticles values(1.92 mg GAE/g);, with all significant value insert ignore into journalissuearticles values(p<0.05);. This study showed that the leaf and other part of Crataegus Azarolus L, rich in total phenolic and total tannin after orbital shaker method was used.
Keywords : Crataegus Azarolus, Tannin, Orbital shaker, Total Phenolic, Leaf