Effects of different combinations of growth regulators on the in vitro growth parameters of Poncirus trifoliata L. (Raf.)

Authors : Elif SUBASİ SEVİNC, Bekir Erol AK, Ibrahim Halil HATİPOGLU, Heydem EKİNCİ

Pages : 598-604

Doi:10.31015/jaefs.2022.4.13

View : 22 | Download : 11

Publication Date : 2022-12-30

Article Type : Research Paper

Abstract :In this study, an in vitro propagation protocol of Poncirus trifoliata L. insert ignore into journalissuearticles values(Raf.); rootstock, which is one of the main rootstocks used in citrus, was created. Monocular node parts of the plant, which is the source of explant, were cultured in MS nutrient media containing BAP at dissmilar concentrations insert ignore into journalissuearticles values(0.5-1.0-2.0 mg/l);. Afterward, it was observed that shoot appearance and tillering were achieved in the node explants cultured in MS nutrient medium comprising 1.0 mg/l BAP. The shoots were removed to a new culture in MS nutrient media containing NAA and IBA for rooting. While 30% rooting was achieved in the rooting medium, 62% of the rooted plantlets were acclimated to external conditions. 2 mg/l BAP and 2 mg/l silver nitrate insert ignore into journalissuearticles values(AgNO3); doses were supplied to the MS medium. The results of this study were obtained and a compound was found to the yellowing and leaf fall problem. As a result, in vitro propagation of Poncirus trifoliata, which is among the important citrus rootstocks, was tried, and its rooting ability was investigated in different nutrient media concentrations after shoots were obtained from different explants.
Keywords : Poncirus trifoliata L, in vitro, silver nitrate, ornamental plant, propagation, tissue culture