Biosynthesis of silver nanoparticles from Arum dioscoridis plant leaf aqueous extract: anticancer and antimicrobial properties

Authors : Polat İPEK, Mehmet Fırat BARAN, Reşit YILDIZ, Abdulkerim HATİPOĞLU

Pages : 399-407

Doi:10.31015/jaefs.2023.2.18

View : 92 | Download : 69

Publication Date : 2023-06-29

Article Type : Research Paper

Abstract :This study was carried out to synthesize silver nanoparticles insert ignore into journalissuearticles values(AgNPs); from Arum dioscoridis insert ignore into journalissuearticles values(AD); leaf extract and to investigate the cytotoxic and antipathogenic effects of them. The plant material had a reducing and stabilizing effect on the synthesized nanomaterial. During the plant-mediated synthesis of nanomaterials, no substances that would cause environmental pollution were used. For the structural characterization of AD-AgNPs, Ultraviolet-visible insert ignore into journalissuearticles values(UV-vis); Spectroscopy, Field Emission Scanning Electron Microscopy insert ignore into journalissuearticles values(FE-SEM);, Electron Dispersive X-ray insert ignore into journalissuearticles values(EDX); Spectroscopy, Fourier Transform Infrared insert ignore into journalissuearticles values(FT-IR); Spectroscopy, Transmission Electron Microscopy insert ignore into journalissuearticles values(TEM);, X-ray Diffractometer insert ignore into journalissuearticles values(XRD);, Atomic Force Microscopy insert ignore into journalissuearticles values(AFM); and Zetasizer analyses were performed. The produced AgNPs showed maximum surface plasmon resonance at 431.67 nm and had mostly spherical morphology. The zeta potential value of the nanomaterial was -9.76 mV and the average powder crystal size was 31.48 nm. The minimum inhibitory concentration insert ignore into journalissuearticles values(MIC); values insert ignore into journalissuearticles values(mg/L); of AD-AgNPs on Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans were 0.25, 2.00, 0.125, 4.00, and 1.00, respectively. After 24 and 48 hours of application by MTT [3-insert ignore into journalissuearticles values(4,5-dimetiltiazol-2-il);-2,5-difeniltetrazolium bromid] assay, the half-maximal inhibitory concentrations insert ignore into journalissuearticles values(IC50: μg/mL); of AD-AgNPs on human colon adenocarcinoma cell insert ignore into journalissuearticles values(CACO-2);, human breast cancer cell insert ignore into journalissuearticles values(MCF-7);, glioblastoma multiforme cell insert ignore into journalissuearticles values(T98-G);, and healthy human umbilical vein endothelial cell insert ignore into journalissuearticles values(HUVEC); lines were determined as 2.977, 2.801, 5.694, 4.392; 2.115, 2.300, 2.612, 4.091, respectively.
Keywords : AgNPs, Arum dioscoridis extract, Cytotoxicity, Green chemistry, Pathogen microorganism

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