Phylogenetic Analysis of Pistacia Species with rbcL Chloroplast Gene Region

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International Journal of Advances in Engineering and Pure Sciences
Volume:35 Issue:3
Phylogenetic Analysis of Pistacia Species with rbcL Chloroplast Gene Region

Phylogenetic Analysis of Pistacia Species with rbcL Chloroplast Gene Region

Pages : 359-367

View : 24 | Download : 23

Publication Date : 2023-09-30

Article Type : Research Paper

Abstract :The phylogeny of five species in the genus Pistacia was assessed using the plastid rbcL gene region. Genomic DNA was isolated from leaf tissue and PCR analysis with primer of rbcL gene region and DNA sequencing were performed. Using obtained sequence data, phylogenetic and PCoA insert ignore into journalissuearticles values(Principle Component Analysis); analyses were performed for reveal the phylogenetic relationships among Pistacia species. According to the result of phylogenetic analysis, closer grouping in clades was observed between P. vera; P. khinjuk and P. atlantica; between P. palaestina and P. terebinthus. Result of PCoA analysis supported the result of phylogenetic analysis. As a result, rbcL gene region was found powerful at species-based grouping. Revealed sequence information of chloroplast gene region is reliable to elaborate a molecular database to conduct breeding programs on local pistachio gene pool.
Keywords : Pistacia, kloroplast geni, rbcL, filogeni

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